Company Makes Globe 100 List for Fourth Year in a Row

American Science and Engineering, Inc. (NASDAQ: ASEI) (AS&E^®), a leading worldwide supplier of innovative X-ray inspection solutions, today announced the Company has been named to The Boston Globe’s “Globe 100” list of top performing public companies in Massachusetts.

Based on a composite score derived from financial data for the four quarters ending December 31, 2008, AS&E was ranked 39th among Massachusetts-based companies whose stock is publicly traded on NASDAQ, the New York Stock Exchange or the American Stock Exchange. It is the fourth time in a row that AS&E has been included in the Globe 100. AS&E was also listed 24th in this year’s “Growth 50” list and 31st in the “Return on Equity” list.

“It is a great honor to be recognized again by The Boston Globe as one of the state’s highest achieving companies and to be listed among such distinguished industry leaders,” said Anthony Fabiano, AS&E’s President and CEO. “We continue to move up in our rankings each year, and are pleased in our fourth year on the list to have also announced just last week outstanding financial and operational results for fiscal year 2009 that include double digit revenue, net income and EPS growth, and the highest revenue in Company history.”

“These companies represent the resiliency of Massachusetts business, navigating difficult waters and surviving the ebbs and flows of an unstable economy and stock market,” said Shirley Leung, business editor of the Boston Globe.

Now in its 21st year, The Globe 100 ranks Massachusetts-based public companies based on financial data from the four quarters ending closest to December 31, 2008, and for corresponding quarters a year earlier. To qualify for the Globe 100, a company must have been public for the entire 2008 calendar year and have reported a positive net income for both 2007 and 2008. To view the 2009 Globe 100, click here: http://www.boston.com/business/globe/globe100/globe_100_2009/globe100/

About the Globe 100 ranking methodology

The Globe 100 ranks Massachusetts-based public companies based on financial data from the four quarters ending closest to December 31, 2008, and for corresponding quarters a year earlier. To be eligible, the company must be traded publicly for the entire 2008 calendar year on the New York Stock Exchange, NASDAQ or the American Stock Exchange and report revenue and profit for both 2007 and 2008.